Gene description for MFGE8
Gene name milk fat globule EGF and factor V/VIII domain containing
Gene symbol MFGE8
Other names/aliases BA46
EDIL1
HMFG
HsT19888
MFG-E8
MFGM
OAcGD3S
SED1
SPAG10
hP47
Species Homo sapiens
 Experiment description of studies that identified MFGE8 in Osteosarcoma cells

1
Experiment ID2024
Identified moleculeprotein
Extracellular vesicle typeLarge extracellular vesicles
MISEVLarge EVs
Identification method Western blotting
PubMed ID 36433666   
OrganismHomo sapiens
Experiment descriptionVascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
AuthorsWhitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM.
Journal name Aging Cell
Publication year2023
SampleOsteosarcoma cells
Sample nameU2OS
Isolation/purification methodsCentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

2
Experiment ID2025
Identified moleculeprotein
Extracellular vesicle typeMedium extracellular vesicles
MISEVLarge EVs
Identification method Western blotting
PubMed ID 36433666   
OrganismHomo sapiens
Experiment descriptionVascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
AuthorsWhitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM.
Journal name Aging Cell
Publication year2023
SampleOsteosarcoma cells
Sample nameU2OS
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID2026
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36433666   
OrganismHomo sapiens
Experiment descriptionVascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
AuthorsWhitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM.
Journal name Aging Cell
Publication year2023
SampleOsteosarcoma cells
Sample nameU2OS
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

<